Electrophoresis, Western Blotting and ELISA

Invitrogen™ NuPAGE™ Antioxidant

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ Mark12™ Unstained Standard

Invitrogen™ Mark12™ Unstained Standard

Allows the closest estimation of molecular weight of your sample protein over a wide molecular weight range

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Cytiva pI Markers

Cytiva pI Markers

Run these markers in parallel with the experimental samples on isoelectric focusing gels. Cytiva Life Sciences™ pI Markers are lyophilized mixtures of stable, salt-free, highly purified proteins.

Thermo Scientific™ Fish Serum Blocking Buffer

Thermo Scientific™ Fish Serum Blocking Buffer

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ Blocker™ Casein

Thermo Scientific™ Blocker™ Casein

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.

Invitrogen™ SimplyBlue™ SafeStain

Invitrogen™ SimplyBlue™ SafeStain

SimplyBlue SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Invitrogen™ eBioscience™ IC Fixation Buffer

Invitrogen™ eBioscience™ IC Fixation Buffer

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Invitrogen™ Novex™ Sharp Pre-stained Protein Standard

Invitrogen™ Novex™ Sharp Pre-stained Protein Standard

Designed for accurate, easy, and convenient molecular weight estimation of a wide range of molecular weight proteins during SDS-PAGE and Western blotting

Thermo Scientific™ Pierce™ Prestained Protein MW Marker

Thermo Scientific™ Pierce™ Prestained Protein MW Marker

A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Afdichttape voor microtiterplaten

Thermo Scientific™ Afdichttape voor microtiterplaten

Clear, self-sticking sealing tape for standard microplates.

Invitrogen™ ELISA TMB Stabilized Chromogen

Invitrogen™ ELISA TMB Stabilized Chromogen

For use with all sandwich ELISAs which utilize Horseradish Peroxidase (HRP) as detecting enzyme

Thermo Scientific™ Pierce™ Silver Stain Kit

Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Thermo Scientific™ Pierce™ stopoplossing voor TMB

Thermo Scientific™ Pierce™ stopoplossing voor TMB

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 4-12%, 1.0 mm, WedgeWell™ format

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 4-12%, 1.0 mm, WedgeWell™ format

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions.

Invitrogen™ SeeBlue™ Plus2 Pre-stained Protein Standard

Invitrogen™ SeeBlue™ Plus2 Pre-stained Protein Standard

Allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 12%, 1.0 mm, WedgeWell™ format

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 12%, 1.0 mm, WedgeWell™ format

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled.

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

Invitrogen™ XCell <i>SureLock</i>™ Mini-Cell

Invitrogen™ XCell SureLock™ Mini-Cell

Quickly and easily run up to two Novex 8 x 8cm minigels, leak-free without any clamps or grease

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 12%, 1.0 mm, WedgeWell™ format

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 12%, 1.0 mm, WedgeWell™ format

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.

BD TMB Substrate Reagent Set

BD TMB Substrate Reagent Set

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.

Thermo Scientific™ 1-Step™ TMB-Blotting substraatoplossing

Thermo Scientific™ 1-Step™ TMB-Blotting substraatoplossing

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

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