Cell Culture Media
Gibco™ Essential 8™ Medium
Essential 8™ Medium
Gibco™ RPMI 1640 Medium (ATCC modification)
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Gibco™ Cell Dissociation Buffer, enzyme-free, PBS
Animal origin-free
Gibco™ DMEM, no glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ N-2 Supplement (100X)
N-2 Supplement (100X)
Gibco™ Advanced DMEM/F-12
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Gibco™ Essential 8™ Flex Medium Kit
Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without the need for weekend feeding. Gibco™ Essential 8™ Flex Medium Kit is provided in a convenient two-component kit (500mL basal medium and 10mL supplement).
Gibco™ MEM
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Gibco™ DMEM/F-12, GlutaMAX™ supplement
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ IMDM, no phenol red
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.
Gibco™ RPMI 1640 Medium, no glucose
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Gibco™ DMEM, no glucose, no glutamine, no phenol red
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol red
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology.
Gibco™ DMEM, high glucose, no glutamine
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ DMEM/F-12
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ IMDM
IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ Ham's F-12 Nutrient Mix
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.
Gibco™ DMEM, high glucose, pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ DMEM, high glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Essential 6™ Medium
Essential 6™ Medium
Gibco™ Ham's F-12K (Kaighn's) Medium
Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.
Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.
Gibco™ RPMI 1640 Medium, HEPES
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Gibco™ MEM α, nucleosides, no ascorbic acid
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Gibco™ DMEM, high glucose, HEPES, no phenol red
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ DMEM/F-12, no phenol red
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Cell culture media support the growth and maintenance of a variety of mammalian and other cell lines in vitro, as either anchorage-dependent or suspension cultures. Cell culture offers convenience, control, consistency and reproducibility of results, especially when using clonal cells.
Cell culture has multiple applications in cellular and molecular biology:
- Studying normal cell physiology and biochemistry
- Industrial bioprocess/bioreactor manufacturing of vaccines, therapeutic proteins and monoclonal antibodies using recombinant, high-yield protein expression systems
- Pharmaceutical screening and development, including drug metabolism and toxicology
- Other emerging applications in stem cell culture, immunotherapy and cell therapy
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
- Classical mammalian cell culture media (DMEM, MEM, RPMI 1640, McCoy’s, Ham’s, BME and EBSS) contain all the nutrients that cells need, such as amino acids, minerals, vitamins and glucose
- Individual cell culture reagents and supplements, including growth factors (e.g. pyruvate), antibiotics, hormones, cryopreservation media, cell dissociation reagents and animal-derived sera (e.g., FBS foetal bovine serum) which contain a ready-made natural mixture of nutrients
- Speciality media, including stem cell culture media, transfection media, and serum-free or animal-derived component-free (ACDF) additives
- Ancillary buffers and process liquids, including balanced salt solutions (PBS phosphate buffered saline, Hank’s HBSS, Earle’s EBBS), sodium bicarbonate and HEPES buffers, phenol red, cell culture grade water, and adherent cell microcarriers
Cell culture media are typically supplied as presterilised ready-to-use liquids, concentrates, powders or other solids in bottles, or else as single-use sealed bags for integration into compatible cell culture harvesting systems.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: