Protein Extraction and Purification

Invitrogen™ eBioscience™ 10X RBC Lysis Buffer (Multi-species)

Invitrogen™ eBioscience™ 10X RBC Lysis Buffer (Multi-species)

Specially formulated for optimal lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues such as spleen. This buffer can be used for lysis of human, mouse, rat, canine, and non-human primate samples.

Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ HisPur™ Ni-NTA Resin

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Cytiva Superose™ 6 Increase Columns

Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes.   Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Thermo Scientific™ Protein Stabilizing Cocktail

Thermo Scientific™ Protein Stabilizing Cocktail

Increase the shelf-life of purified or partially purified proteins during routine storage using this versatile stabilizing solution.

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Invitrogen™ DynaMag™-15 Magnet

Invitrogen™ DynaMag™-15 Magnet

Optimized for efficient cell isolation using all types of Dynabeads™ (range 1–4.5 μm in diameter) in standard 15 mL lab tubes.

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.38 MDL Number: MFCD00036175 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O

Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Cytiva HisTrap™ HP Prepacked Columns

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Thermo Scientific™ Pierce™ Disposable Columns, 10 mL

Thermo Scientific™ Pierce™ Disposable Columns, 10 mL

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Thermo Scientific™ Pierce™ Protein Concentrators PES, 3K or 5K MWCO, 0.5–100 mL

Thermo Scientific™ Pierce™ Protein Concentrators PES, 3K or 5K MWCO, 0.5–100 mL

Thermo Scientific Pierce Protein Concentrators PES are easy-to-use centrifugal devices that provide fast processing and excellent recovery of proteins with MWCO values of 3K and 5K, in 0.5–100 mL sample volumes.

Thermo Scientific™ B-PER™ Reagent (in Phosphate Buffer)

Thermo Scientific™ B-PER™ Reagent (in Phosphate Buffer)

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Sodium Deoxycholate Detergent

Thermo Scientific™ Sodium Deoxycholate Detergent

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Thermo Scientific™ PMSF Protease Inhibitor

Thermo Scientific™ PMSF Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Invitrogen™ Magnetic Stand-96

Invitrogen™ Magnetic Stand-96

The stand has clear polyacryl base in standard SBS 96-well microplate footprint and uses 24 extremely strong permanent magnets for fast, easy magnetic separation in little as 30 seconds.

Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Subcellular Protein Fractionation Kit for Tissues

Thermo Scientific™ Pierce™ Centrifuge Columns, 5 mL

Thermo Scientific™ Pierce™ Centrifuge Columns, 5 mL

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

Thermo Scientific™ Low Protein Binding Microcentrifuge Tubes

Thermo Scientific™ Low Protein Binding Microcentrifuge Tubes

Minimize protein sample loss during sample manipulation, especially when there are extensive processing steps, by using Thermo Scientific Pierce Low Protein Binding Microcentrifuge Tubes.

Cytiva HiTrap Capto Butyl ImpRes

Cytiva HiTrap Capto Butyl ImpRes

HiTrap Capto Butyl ImpRes is a ready-to-use column prepacked with BioProcess Capto Butyl ImpRes resins for screening and small-scale protein purification using hydrophobic interaction chromatography.

Cytiva HisTrap™ FF Crude Prepacked Columns

Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

MP Biomedicals™ FastPROTEIN™ Red Matrix

MP Biomedicals™ FastPROTEIN™ Red Matrix

FastPROTEIN™ Red Matrix Kit designed for the rapid extraction of proteins from yeast cells, fungi and algea in a few seconds.

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000.  Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

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